Abstract
Hybridomas between spleen cells from BALB/c mice and Sp2/O-Ag14 mycloma cell line are performed by PEG fusion. An in vitro booster is realized with human thymic reticulo-epithelial cell cultures as antigen. A monoclonal antibody (3H9) directed against the thymic epithelium and stratified epithelia of mammals has been obtained and characterized.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Epithelium / immunology
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Female
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Humans
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Hybridomas / immunology
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Immunoglobulin M / immunology
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Immunoglobulin kappa-Chains / immunology
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Mammals / immunology
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Mice
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Mice, Inbred BALB C / immunology
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Organ Specificity
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Species Specificity
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Thymus Gland / immunology*
Substances
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Antibodies, Monoclonal
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Immunoglobulin M
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Immunoglobulin kappa-Chains