Measuring extracellular pH in a lung fibrosis model with acidoCEST MRI

Mol Imaging Biol. 2015 Apr;17(2):177-84. doi: 10.1007/s11307-014-0784-6.

Abstract

Purpose: A feed-forward loop involving lactic acid production may potentially occur during the formation of idiopathic pulmonary fibrosis. To provide evidence for this feed-forward loop, we used acidoCEST MRI to measure the extracellular pH (pHe), while also measuring percent uptake of the contrast agent, lesion size, and the apparent diffusion coefficient (ADC).

Procedures: We developed a respiration-gated version of acidoCEST MRI to improve the measurement of pHe and percent uptake in lesions. We also used T2-weighted MRI to measure lesion volumes and diffusion-weighted MRI to measure ADC.

Results: The longitudinal changes in average pHe and % uptake of the contrast agent were inversely related to reduction in lung lesion volume. The average ADC did not change during the time frame of the study.

Conclusions: The increase in pHe during the reduction in lesion volume indicates a role for lactic acid in the proposed feed-forward loop of IPF.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / metabolism
  • Computer Simulation
  • Disease Models, Animal
  • Extracellular Space / chemistry*
  • Hydrogen-Ion Concentration
  • Idiopathic Pulmonary Fibrosis / metabolism*
  • Iopamidol / chemistry
  • Iopamidol / pharmacology
  • Lung / pathology
  • Magnetic Resonance Imaging / methods*
  • Male
  • Mice, Inbred C57BL
  • Respiration

Substances

  • Collagen
  • Iopamidol