Evaluation of the novel microarray-based Prove-it™ Bone&Joint assay for direct detection of pathogens from normally sterile body sites in comparison with culture and broad-range bacterial PCR

Vladimira Hinić; Anne Aittakorpi; Sylvia Suter; Selda Turan; Elisabeth Schultheiss; Reno Frei; Daniel Goldenberger

doi:10.1016/j.mimet.2014.08.004

Evaluation of the novel microarray-based Prove-it™ Bone&Joint assay for direct detection of pathogens from normally sterile body sites in comparison with culture and broad-range bacterial PCR

J Microbiol Methods. 2014 Dec:107:38-40. doi: 10.1016/j.mimet.2014.08.004. Epub 2014 Sep 4.

Authors

Vladimira Hinić¹, Anne Aittakorpi², Sylvia Suter³, Selda Turan³, Elisabeth Schultheiss³, Reno Frei³, Daniel Goldenberger³

Affiliations

¹ Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland. Electronic address: [email protected].
² Mobidiag Ltd., Helsinki, Finland.
³ Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.

PMID: 25194235
DOI: 10.1016/j.mimet.2014.08.004

Abstract

The performance of Prove-it™ Bone&Joint, a novel microarray-based assay for direct pathogen detection from clinical samples was assessed. In culture-positive samples, Prove-it™ performed similarly with broad-range bacterial PCR in osteoarticular (sensitivity 62.9% vs. 57.7%) and non-osteoarticular samples (54.6% vs. 56.8%). Prove-it™ is a rapid tool providing preliminary results while awaiting culture results.

Keywords: Broad-range bacterial PCR; Culture; Direct molecular detection; Microarray; Osteoarticular and other specimens.

MeSH terms

Arthritis, Infectious / diagnosis*
Arthritis, Infectious / microbiology*
Bacteria / classification
Bacteria / genetics*
Bacteria / isolation & purification
Humans
Osteitis / diagnosis*
Osteitis / microbiology*
Polymerase Chain Reaction / methods*
Reproducibility of Results
Sensitivity and Specificity