The protective effects of curcumin on experimental acute liver lesion induced by intestinal ischemia-reperfusion through inhibiting the pathway of NF-κB in a rat model

Oxid Med Cell Longev. 2014:2014:191624. doi: 10.1155/2014/191624. Epub 2014 Aug 20.

Abstract

Objective: In this study, we investigated the protective effect and mechanism of curcumin on a rat model of intestinal ischemia/reperfusion (I/R), which induces an acute liver lesion.

Methods: Curcumin was injected into rats in the curcumin groups through left femoral vein. The same volume of vehicle (0.9% normal saline) was injected into sham and I/R groups. Blood and liver tissue were gathered for serological and histopathological determination.

Results: Intestinal I/R led to severe liver injury manifested as a significant increase in serum AST and ALT levels; all of those were reduced by treatment with curcumin. Simultaneously, the activity of SOD in liver decreased after intestinal I/R, which was increased by curcumin treatment. On the other hand, curcumin reduced MPO activity of liver tissue, as well as serum IL-6 and TNF-α levels observably. This is in parallel with the decreased level of liver intercellular cell adhesion molecule-1 (ICAM-1) and nuclear factor-κB (NF-κB) expression.

Conclusion: Our findings suggest that curcumin treatment attenuates liver lesion induced by intestinal I/R, attributable to the antioxidative and anti-inflammatory effect via inhibition of the NF-κB pathway.

MeSH terms

  • Acute Disease
  • Alanine Transaminase / blood
  • Animals
  • Aspartate Aminotransferases / blood
  • Curcumin / pharmacology*
  • Disease Models, Animal
  • Intercellular Adhesion Molecule-1 / metabolism
  • Interleukin-6 / metabolism
  • Liver / drug effects
  • Liver / metabolism
  • Liver / pathology
  • Liver Diseases / etiology
  • Liver Diseases / pathology
  • Liver Diseases / prevention & control
  • Male
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism*
  • Peroxidase / metabolism
  • Protective Agents / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / complications
  • Reperfusion Injury / pathology
  • Signal Transduction / drug effects*
  • Superoxide Dismutase / metabolism

Substances

  • Interleukin-6
  • NF-kappa B
  • Protective Agents
  • Intercellular Adhesion Molecule-1
  • Peroxidase
  • Superoxide Dismutase
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Curcumin