The activity of hypothalamic neuroendocrine system is pivotal in the regulation of hydromineral balance, cardiovascular activity, parturition, lactation and others. The activity of this system is closely associated with the interactions between astrocytes and neuroendocrine neurons. Based on recent experimental discoveries, this review will systematically analyze roles of supraoptic astrocytes in the effects of osmotic and suckling stimuli on vasopressin and oxytocin neuron activity in the hypothalamus, and then discuss the mechanisms underlying these actions.