Three new anthranilic acid derivatives: N-(R)-3'-hydroxydocosanoylanthranilic acid (1), N-(R)-3'-hydroxytricosanoylanthranilic acid (2) and N-(R)-3'-hydroxytetracosanoylanthranilic acid (3), in addition to one knownanthranilic acid and six known flavonoids, were isolated from the ethyl acetate extract of Ononis pusilla L. The structures of the isolated compounds were assigned by spectroscopic methods, including 1D and 2D homo and heteronuclear NMR experiments, ESI-MS, chemical transformation and comparison with literature data.