Determination of phosphoglucomutase (PGM1), acid phosphatase (ACP), and esterase D (ESD) in human bloodstains by hybrid isoelectric focusing (HIEF)

I Muñoz-Barús; M V Lareu; I López-Rodriguez; M S Rodriguez-Calvo; A Carracedo

doi:10.1007/BF00201323

Determination of phosphoglucomutase (PGM1), acid phosphatase (ACP), and esterase D (ESD) in human bloodstains by hybrid isoelectric focusing (HIEF)

Z Rechtsmed. 1989;102(4):271-5. doi: 10.1007/BF00201323.

Authors

I Muñoz-Barús¹, M V Lareu, I López-Rodriguez, M S Rodriguez-Calvo, A Carracedo

Affiliation

¹ Department of Legal Medicine, Faculty of Medicine, University of Santiago, La Coruña, Spain.

PMID: 2523625
DOI: 10.1007/BF00201323

Abstract

PGM1, ESD, and ACP were determined in bloodstain extracts by isoelectric focusing (IEF) with carrier ampholytes (CA) and HIEF. HIEF yields superior results in PGM typing from bloodstain extracts, whereas for ESD and ACP typing isoelectric focusing with carrier ampholytes seems to be the method of choice.

MeSH terms

Acid Phosphatase / genetics*
Blood Grouping and Crossmatching
Blood Stains*
Carboxylesterase*
Carboxylic Ester Hydrolases / genetics*
Humans
Isoelectric Focusing*
Isoenzymes / genetics*
Phenotype
Phosphoglucomutase / genetics*
Polymorphism, Genetic

Substances

Isoenzymes
Carboxylic Ester Hydrolases
Carboxylesterase
ESD protein, human
Acid Phosphatase
Phosphoglucomutase