Evaluation of a rapid antigen detection point-of-care test for respiratory syncytial virus and influenza in a pediatric hospitalized population in the Netherlands

Andrea H L Bruning; Karin van Dijk; Hetty W M van Eijk; Gerrit Koen; Job B M van Woensel; Frea H Kruisinga; Dasja Pajkrt; Katja C Wolthers

doi:10.1016/j.diagmicrobio.2014.08.010

Evaluation of a rapid antigen detection point-of-care test for respiratory syncytial virus and influenza in a pediatric hospitalized population in the Netherlands

Diagn Microbiol Infect Dis. 2014 Dec;80(4):292-3. doi: 10.1016/j.diagmicrobio.2014.08.010. Epub 2014 Aug 30.

Authors

Andrea H L Bruning¹, Karin van Dijk², Hetty W M van Eijk³, Gerrit Koen³, Job B M van Woensel⁴, Frea H Kruisinga⁴, Dasja Pajkrt⁴, Katja C Wolthers³

Affiliations

¹ Department of Pediatrics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. Electronic address: [email protected].
² Department of Medical Microbiology and Infection Control, VU University Medical Center, De Boelelaan 1118, 1081 HZ Amsterdam, The Netherlands.
³ Laboratory of Clinical Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
⁴ Department of Pediatrics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.

PMID: 25241640
DOI: 10.1016/j.diagmicrobio.2014.08.010

Abstract

This pilot study evaluates the diagnostic performance of Sofia RSV Fluorescent Immunoassay Analyzer (FIA) and Sofia Influenza A + B FIA for rapid detection of respiratory syncytial virus and influenza A and B. Sofia had a lower-than-expected sensitivity for all viruses and a high rate of false-positive results for influenza B virus.

Keywords: Antigen detection; Children; Point-of-care; Rapid testing; Respiratory infection.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Adolescent
Antigens, Viral / analysis
Child
Child, Preschool
False Positive Reactions
Fluoroimmunoassay / instrumentation
Fluoroimmunoassay / methods*
Humans
Infant
Infant, Newborn
Influenza A virus / immunology
Influenza A virus / pathogenicity
Influenza B virus / immunology
Influenza B virus / pathogenicity
Influenza, Human / diagnosis
Influenza, Human / virology*
Netherlands
Pilot Projects
Point-of-Care Systems
Polymerase Chain Reaction / methods
Respiratory Syncytial Virus Infections / diagnosis*
Respiratory Syncytial Virus Infections / virology
Respiratory Syncytial Virus, Human / immunology*
Respiratory Syncytial Virus, Human / pathogenicity
Respiratory Tract Infections / diagnosis
Respiratory Tract Infections / virology
Sensitivity and Specificity

Substances

Antigens, Viral