Abstract
We studied the response to thymus-independent type 2 (type 2) Ag in mice suppressed from birth with anti-Ia antibody. Although these mice have significantly reduced numbers of surface IgM+ cells and reduced or absent levels of Ia-restricted Th cell activity, their IgM antibody response to the type 2 Ag TNP-Ficoll was unaffected whereas that to the prototypic thymus-dependent Ag SRBC was predictably eliminated. These data suggest that an in vivo antibody response can be made to type 2 Ag in the absence of Ia-dependent cellular interactions. The surface IgM+IgD-Ia- B cells that are found in the anti-Ia antibody-suppressed mouse may represent an expanded population of Ia-independent, type 2 Ag-sensitive B cells normally present as a smaller proportion of the splenic lymphocyte population. Thymus-dependent responses, which have been shown to have an absolute requirement for an Ia-dependent interaction, are absent in these animals.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn / immunology*
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Antibodies, Monoclonal / administration & dosage*
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Antigens, T-Independent / administration & dosage*
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Antigens, T-Independent / immunology
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B-Lymphocytes / metabolism
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Cell Communication
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Ficoll / administration & dosage*
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Ficoll / analogs & derivatives
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Ficoll / immunology
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Hemolytic Plaque Technique
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Histocompatibility Antigens Class II / immunology*
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Immunosuppressive Agents / administration & dosage*
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Lymphocyte Culture Test, Mixed
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Mice
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Mice, Inbred C3H
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Nitrobenzenes / administration & dosage*
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Polysaccharides / administration & dosage*
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Trinitrobenzenes / administration & dosage*
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Trinitrobenzenes / immunology
Substances
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Antibodies, Monoclonal
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Antigens, T-Independent
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Histocompatibility Antigens Class II
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Immunosuppressive Agents
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Nitrobenzenes
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Polysaccharides
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TNP-ficoll
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Trinitrobenzenes
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Ficoll