Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis

EMBO Rep. 2014 Nov;15(11):1202-9. doi: 10.15252/embr.201438660. Epub 2014 Sep 26.

Abstract

Cell-to-cell communication is a fundamental mechanism for coordinating developmental and physiological events in multicellular organisms. Heterotrimeric G proteins are key molecules that transmit extracellular signals; similarly, CLAVATA signaling is a crucial regulator in plant development. Here, we show that Arabidopsis thaliana Gβ mutants exhibit an enlarged stem cell region, which is similar to that of clavata mutants. Our genetic and cell biological analyses suggest that the G protein beta-subunit1 AGB1 and RPK2, one of the major CLV3 peptide hormone receptors, work synergistically in stem cell homeostasis through their physical interactions. We propose that AGB1 and RPK2 compose a signaling module to facilitate meristem development.

Keywords: Arabidopsis thaliana; RECEPTOR‐LIKE PROTEIN KINASE 2; heterotrimeric G protein; peptide hormone; stem cell homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cambium / metabolism*
  • Cambium / physiology
  • Cell Proliferation*
  • GTP-Binding Protein beta Subunits / genetics
  • GTP-Binding Protein beta Subunits / metabolism*
  • Protein Binding
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Signal Transduction

Substances

  • AGB1 protein, Arabidopsis
  • AT2G27250 protein, Arabidopsis
  • Arabidopsis Proteins
  • GTP-Binding Protein beta Subunits
  • Protein Serine-Threonine Kinases
  • receptor-like protein kinase 2, Arabidopsis