Abstract
The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5'-terminal 900 nucleotides and 3' untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3' UTR and 5'-terminal region of RNA1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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5' Untranslated Regions / genetics
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Amino Acid Sequence
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Base Sequence
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Genome, Viral / genetics*
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Molecular Sequence Data
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Nepovirus / classification
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Nepovirus / genetics*
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Nepovirus / isolation & purification
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Phylogeny
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Plant Diseases / virology
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Prunus / virology*
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RNA, Viral / genetics
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Reassortant Viruses / classification
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Reassortant Viruses / genetics*
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Reassortant Viruses / isolation & purification
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Recombination, Genetic*
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Rubus / virology*
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Sequence Alignment
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Sequence Analysis, RNA
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Sequence Homology, Amino Acid
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Viral Proteins / genetics
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Vitis / virology*
Substances
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3' Untranslated Regions
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5' Untranslated Regions
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RNA, Viral
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Viral Proteins
Associated data
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GENBANK/KM083890
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GENBANK/KM083891
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GENBANK/KM083892
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GENBANK/KM083893
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GENBANK/KM083894
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GENBANK/KM083895