Multifunctional RNA nanoparticles

Nano Lett. 2014 Oct 8;14(10):5662-71. doi: 10.1021/nl502385k. Epub 2014 Sep 30.

Abstract

Our recent advancements in RNA nanotechnology introduced novel nanoscaffolds (nanorings); however, the potential of their use for biomedical applications was never fully revealed. As presented here, besides functionalization with multiple different short interfering RNAs for combinatorial RNA interference (e.g., against multiple HIV-1 genes), nanorings also allow simultaneous embedment of assorted RNA aptamers, fluorescent dyes, proteins, as well as recently developed RNA-DNA hybrids aimed to conditionally activate multiple split functionalities inside cells.

Keywords: RNA interference; RNA nanoparticles; RNA nanotechnology; RNA−DNA hybrid reassociation; aptamers.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Female
  • Genetic Therapy
  • HIV Infections / therapy
  • HIV Infections / virology
  • HIV-1 / genetics
  • Humans
  • Mice, Nude
  • Models, Molecular
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Neoplasms / genetics
  • Neoplasms / therapy
  • RNA Interference*
  • RNA, Small Interfering / administration & dosage*
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / therapeutic use*

Substances

  • RNA, Small Interfering