Abstract
MafA and MafB are basic leucine zipper transcription factors expressed in mature pancreatic β- and α-cells, respectively. MafA is not only an insulin gene transcription factor but is also critical for the maturation and maintenance of β-cell function, whereas MafB is expressed in immature β-cells during development and in compromised β-cells in diabetes. In this study, we developed a mouse model to easily trace the promoter activity of MafA in β-cells as a tool for studying β-cell differentiation, maturation, regeneration and function using the expression of the fluorescent protein Kusabira Orange (KOr) driven by the BAC-mafA promoter. The expression of KOr was highly restricted to β-cells in the transgenic pancreas. By crossing MafA-KOr mice with MafB(GFP/+) reporter mice, simultaneous monitoring of MafA and MafB expressions in the isolated islets was successfully performed. This system can be a useful tool for examining dynamic changes in the differentiation and function of pancreatic islets by visualizing the expressions of MafA and MafB.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biomarkers / metabolism
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Cell Differentiation*
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Crosses, Genetic
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Embryo, Mammalian / cytology
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Embryo, Mammalian / metabolism
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Gene Expression Regulation
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Genes, Reporter
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Insulin / blood
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Insulin / metabolism
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Insulin Secretion
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Insulin-Secreting Cells / cytology
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Insulin-Secreting Cells / metabolism*
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Luminescent Agents / metabolism*
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism*
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Maf Transcription Factors, Large / genetics
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Maf Transcription Factors, Large / metabolism*
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MafB Transcription Factor / genetics
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MafB Transcription Factor / metabolism*
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Male
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Organ Specificity
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Promoter Regions, Genetic*
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Recombinant Proteins / metabolism
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Red Fluorescent Protein
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Tissue Culture Techniques
Substances
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Biomarkers
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Insulin
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Luminescent Agents
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Luminescent Proteins
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Maf Transcription Factors, Large
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MafB Transcription Factor
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Mafa protein, mouse
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Mafb protein, mouse
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Recombinant Proteins
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Green Fluorescent Proteins