Musculoskeletal Atrophy in an Experimental Model of Knee Osteoarthritis: The Effects of Exercise Training and Low-Level Laser Therapy

Am J Phys Med Rehabil. 2015 Aug;94(8):609-16. doi: 10.1097/PHM.0000000000000219.

Abstract

Objective: The aim of this study was to evaluate the effects of an exercise training protocol and low-level laser therapy (and the association of both treatments) on musculoskeletal atrophy using an experimental model of knee osteoarthritis (OA).

Design: Fifty male Wistar rats were randomly divided into five groups: control group, knee OA control group, OA plus exercise training group, OA plus low-level laser therapy group, and OA plus exercise training associated with low-level laser therapy group. The exercise training and the laser irradiation started 4 wks after the surgery, 3 days per week for 8 wks. The exercise was performed at a speed of 16 m/min, 3 days per week, 50 mins per day, for 8 wks. Laser irradiation was applied at two points of the left knee joint (medial and lateral), for 24 sessions.

Results: The results showed that both trained groups (irradiated or not) presented a significant increase in the muscle cross-sectional area and a decrease in muscle fiber density compared with the knee OA control group. Moreover, both trained and laser-irradiated groups demonstrated decreased muscle-specific ring-finger protein 1 and atrogin-1 immunoexpression.

Conclusions: These results suggest that exercise training and low-level laser therapy were effective in preventing musculoskeletal alterations related to atrophy caused by the degenerative process induced by knee OA.

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Combined Modality Therapy
  • Immunohistochemistry
  • Low-Level Light Therapy*
  • Male
  • Microscopy
  • Models, Animal
  • Muscle Fibers, Skeletal / metabolism
  • Muscle Fibers, Skeletal / pathology
  • Muscle Proteins / metabolism
  • Muscle, Skeletal / pathology
  • Muscular Atrophy / metabolism
  • Muscular Atrophy / pathology
  • Muscular Atrophy / prevention & control*
  • Osteoarthritis, Knee / complications*
  • Physical Conditioning, Animal*
  • Polycomb Repressive Complex 1 / metabolism
  • Random Allocation
  • Rats, Wistar
  • SKP Cullin F-Box Protein Ligases / metabolism
  • Ubiquitin-Protein Ligases / metabolism

Substances

  • Muscle Proteins
  • Fbxo32 protein, mouse
  • Polycomb Repressive Complex 1
  • Ring1 protein, rat
  • SKP Cullin F-Box Protein Ligases
  • Ubiquitin-Protein Ligases