Abstract
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a pattern-recognition receptor for a variety of endogenous and exogenous ligands. However, LOX-1 function in the host immune response is not fully understood. Here, we report that LOX-1 expressed on dendritic cells (DCs) and B cells promotes humoral responses. On B cells LOX-1 signaling upregulated CCR7, promoting cellular migration toward lymphoid tissues. LOX-1 signaling on DCs licensed the cells to promote B cell differentiation into class-switched plasmablasts and led to downregulation of chemokine receptor CXCR5 and upregulation of chemokine receptor CCR10 on plasmablasts, enabling their exit from germinal centers and migration toward local mucosa and skin. Finally, we found that targeting influenza hemagglutinin 1 (HA1) subunit to LOX-1 elicited HA1-specific protective antibody responses in rhesus macaques. Thus, LOX-1 expressed on B cells and DC cells has complementary functions to promote humoral immune responses.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibody Formation / immunology
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B-Lymphocytes / immunology*
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Cell Differentiation / immunology
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Cell Movement / immunology
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Dendritic Cells / immunology*
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Germinal Center / cytology
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Humans
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Immunoglobulin A / biosynthesis
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Immunoglobulin Class Switching / immunology*
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Immunoglobulin G / biosynthesis
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Immunoglobulin M / biosynthesis
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Lymphocyte Activation / immunology
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Macaca mulatta
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Male
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Mucous Membrane / cytology
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Receptors, CCR10 / biosynthesis
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Receptors, CCR7 / biosynthesis
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Receptors, CXCR5 / biosynthesis
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Scavenger Receptors, Class E / biosynthesis
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Scavenger Receptors, Class E / immunology*
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Signal Transduction / immunology
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Skin / cytology
Substances
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CCR10 protein, human
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CCR7 protein, human
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CXCR5 protein, human
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H1N1 virus hemagglutinin
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Hemagglutinin Glycoproteins, Influenza Virus
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Immunoglobulin A
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Immunoglobulin G
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Immunoglobulin M
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OLR1 protein, human
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Receptors, CCR10
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Receptors, CCR7
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Receptors, CXCR5
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Scavenger Receptors, Class E