Programmable protein-DNA hybrid hydrogels for the immobilization and release of functional proteins

Yuzhou Wu; Chuang Li; Felix Boldt; Yanran Wang; Seah Ling Kuan; Thuy Tam Tran; Viktoria Mikhalevich; Christina Förtsch; Holger Barth; Zhongqiang Yang; Dongsheng Liu; Tanja Weil

doi:10.1039/c4cc07144a

Programmable protein-DNA hybrid hydrogels for the immobilization and release of functional proteins

Chem Commun (Camb). 2014 Dec 4;50(93):14620-2. doi: 10.1039/c4cc07144a.

Authors

Yuzhou Wu¹, Chuang Li, Felix Boldt, Yanran Wang, Seah Ling Kuan, Thuy Tam Tran, Viktoria Mikhalevich, Christina Förtsch, Holger Barth, Zhongqiang Yang, Dongsheng Liu, Tanja Weil

Affiliation

¹ Institute of Organic Chemistry III, Macromolecular Chemistry and Biomaterials, University of Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany. [email protected] [email protected].

PMID: 25311614
DOI: 10.1039/c4cc07144a

Abstract

A modular approach for the precise assembly of multi-component hydrogels consisting of protein and DNA building blocks is described for the first time. Multi-arm DNA is designed for crosslinking and stepwise, non-covalent assembly of active proteins inside the hydrogel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival
Cells, Cultured
DNA / chemistry*
Humans
Hydrogels / chemistry*
Immobilized Proteins / chemistry*
Immobilized Proteins / metabolism*
Models, Molecular

Substances

Hydrogels
Immobilized Proteins
DNA