Abstract
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / genetics
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Antibody Specificity
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Antigen-Antibody Reactions
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Bacteriophage lambda / genetics*
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Base Sequence
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Cloning, Molecular / methods
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Escherichia coli / genetics
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Gene Amplification
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Gene Library
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Genetic Vectors*
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Hemocyanins / analogs & derivatives
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Hemocyanins / immunology
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Immunoglobulin Fab Fragments / biosynthesis
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Immunoglobulin Fragments / biosynthesis*
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Immunoglobulin Fragments / genetics
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Mice
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Molecular Sequence Data
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Organophosphorus Compounds / immunology
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
Substances
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Antibodies, Monoclonal
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Immunoglobulin Fab Fragments
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Immunoglobulin Fragments
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Organophosphorus Compounds
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Recombinant Proteins
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keyhole limpet hemocyanin phosphonamidate conjugate
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Hemocyanins