Differential sensing of MAP kinases using SOX-peptides

Angew Chem Int Ed Engl. 2014 Dec 15;53(51):14064-8. doi: 10.1002/anie.201408256. Epub 2014 Oct 15.

Abstract

Five SOX peptides are used to classify the MAPK groups and isoforms thereof using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis leads to the quantification of ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross-reactivity among the peptides, and some unexpected surprises.

Keywords: biosensors; chemometrics; differential sensing; fluorescent probes; mitogen-activated protein kinases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • Mitogen-Activated Protein Kinases / chemistry*
  • Mitogen-Activated Protein Kinases / metabolism
  • Molecular Structure
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Sulfonamides / chemistry
  • Sulfonamides / pharmacology*

Substances

  • Peptides
  • Sulfonamides
  • Mitogen-Activated Protein Kinases