Analysis of the heavy-chain modification and TSG-6 activity in pathological hyaluronan matrices

Methods Mol Biol. 2015:1229:543-8. doi: 10.1007/978-1-4939-1714-3_42.

Abstract

During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.

MeSH terms

  • Asthma / pathology
  • Blotting, Western
  • Cell Adhesion Molecules / metabolism*
  • Colitis, Ulcerative / pathology
  • Colon / metabolism
  • Colon / pathology*
  • Humans
  • Hyaluronic Acid / metabolism*
  • Lung / metabolism
  • Lung / pathology*

Substances

  • Cell Adhesion Molecules
  • TNFAIP6 protein, human
  • Hyaluronic Acid