Invariant natural killer T (iNKT) cells in HAART-treated, HIV-positive patients with bone and cardiovascular impairment

PLoS One. 2014 Oct 17;9(10):e110287. doi: 10.1371/journal.pone.0110287. eCollection 2014.

Abstract

Background: Invariant Natural Killer T (iNKT) cells represent a determinant in the course of infections and diseases, however, their role in the pathogenesis of non-infectious co-morbidities in HIV-positive patients is unknown.

Methods: Flow cytometry was used to investigate iNKT cell frequency, phenotype and function in HIV-infected patients on HAART with bone and/or cardiovascular disorders and in HIV-positive controls free from co-morbidities.

Results: iNKT cells from subjects with bone and cardiovascular impairment expressed high levels of CD161 and predominantly secreted TNF. iNKT cells from individuals with bone disease alone did not show any distinctive phenotypical or functional characteristics. The functional capacity of iNKT cells in patients with cardiovascular disorder was impaired with no cytokine release upon stimulation.

Conclusion: iNKT cells may have a role in non-infectious co-morbidities in treated HIV disease, possibly through the exacerbation of inflammation. Further studies are needed to investigate iNKT cells in the pathogenesis of non-communicable disorders in HIV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antiretroviral Therapy, Highly Active*
  • Bone Diseases / complications*
  • Bone Diseases / immunology
  • Cardiovascular Diseases / complications*
  • Cardiovascular Diseases / immunology
  • Case-Control Studies
  • Female
  • HIV Infections / complications
  • HIV Infections / drug therapy
  • HIV Infections / immunology*
  • Humans
  • Killer Cells, Natural / metabolism*
  • Male
  • Middle Aged
  • NK Cell Lectin-Like Receptor Subfamily B / genetics
  • NK Cell Lectin-Like Receptor Subfamily B / metabolism

Substances

  • NK Cell Lectin-Like Receptor Subfamily B

Grants and funding

Grants from Italian Ministry of Health (GR-2009-1592029) and the Fellowship Program 2012 (9Ab87b2822). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.