Identification of microRNAs in human plasma

Bram C van der Eerden; Rodrigo D Alves; Christel E Kockx; Zeliha Ozgur; Marijke Schreuders-Koedam; Jeroen van de Peppel; Wilfred F van Ijcken; Johannes P van Leeuwen

doi:10.1007/978-1-4939-1619-1_7

Identification of microRNAs in human plasma

Methods Mol Biol. 2015:1226:71-85. doi: 10.1007/978-1-4939-1619-1_7.

Authors

Bram C van der Eerden¹, Rodrigo D Alves, Christel E Kockx, Zeliha Ozgur, Marijke Schreuders-Koedam, Jeroen van de Peppel, Wilfred F van Ijcken, Johannes P van Leeuwen

Affiliation

¹ Department of Internal Medicine, Erasmus MC, Room Ee585b, 3000 DR, 1738, Rotterdam, The Netherlands, [email protected].

PMID: 25331044
DOI: 10.1007/978-1-4939-1619-1_7

Abstract

In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
MicroRNAs* / blood
MicroRNAs* / genetics
MicroRNAs* / isolation & purification
Sequence Analysis, RNA / methods*

Substances

MicroRNAs