Comparison of oxidative DNA damage between alcohol-dependent patients with and without delirium tremens

Alcohol Clin Exp Res. 2014 Oct;38(10):2523-8. doi: 10.1111/acer.12539. Epub 2014 Oct 21.

Abstract

Background: Chronic and excessive alcohol consumption increases oxidative stress. We previously found that levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, are elevated in alcohol-dependent patients without delirium tremens (DTs). The aim of this study was to compare serum 8-OHdG levels between alcohol-dependent patients with and without DTs.

Methods: We recruited 16 alcohol-dependent patients with DTs (DTs group) and 58 patients without DTs (non-DTs group). Alcohol withdrawal severity was evaluated using the Chinese version of the revised Clinical Institute Withdrawal Assessment for Alcohol Scale (CIWA-Ar-C) every 8 hours. Serum levels of 8-OHdG and other biological indices were assayed at baseline and after 1 week of detoxification.

Results: The mean 8-OHdG level in the DTs group was significantly higher than that in the non-DTs group (0.50 vs. 0.34 ng/ml, p < 0.001). A significant correlation was found between the highest CIWA-Ar-C scores and serum 8-OHdG levels (β = 0.43, p = 0.001) in the non-DTs group, but not in the DTs group (β = 0.34, p = 0.19). An area under receiver operating characteristic curve of 0.83 suggests that 8-OHdG levels potentially differentiate patients with DTs from those without DTs. After dividing the patients into quartiles by 8-OHdG level, we found that compared to the patients in the third and fourth quartiles, the patients in the highest quartile had an odds ratio of 24.1 (p < 0.001) to have DTs, while those in the second highest quartile had an odds ratio of 3.5 (p = 0.19). Serum 8-OHdG levels did not significantly change after 1 week of detoxification in either group.

Conclusions: Alcohol-dependent patients with DTs have higher serum 8-OHdG levels than those without DTs, suggesting that higher oxidative stress carries a greater risk of the occurrence of DTs.

Keywords: 8-Hydroxy-2′-Deoxyguanosine; Alcohol Dependence; Alcohol Withdrawal; Delirium Tremens; Oxidative Stress.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Alcohol Withdrawal Delirium / blood
  • Alcohol Withdrawal Delirium / epidemiology
  • Alcohol Withdrawal Delirium / physiopathology*
  • Alcoholics*
  • Alcoholism / blood
  • Alcoholism / epidemiology
  • Alcoholism / physiopathology*
  • Biomarkers / blood
  • Comorbidity
  • DNA Damage / physiology*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / blood
  • Female
  • Humans
  • Male
  • Middle Aged
  • Oxidative Stress / physiology*
  • Risk Factors

Substances

  • Biomarkers
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine