Abstract
Esophageal squamous cell carcinoma (ESCC) is a common cancer with poor prognosis. In order to identify useful biomarkers for accurately classifying prognostic risks for ESCC patients, we examined the expression of six proteins by immunohistochemistry (IHC) in 590 paraffin-embedded ESCC samples. The candidate proteins include p53, EGFR, c-KIT, TIMP1 and PI3K-p110α reported to be altered in ESCC tissues as well as another important component of PI3K, PI3K-p85α. Of the six proteins tested, p53, EGFR, c-KIT, TIMP1 and PI3K-p85α were detected with high expression in 43.0%, 36.6%, 55.9%, 70.7% and 57.1% of tumors, respectively. Significant associations were found between high expression of PI3K-p85α, EGFR and p53 and poor prognosis (P = 0.00111; 0.00001; 0.00426). Applying these three proteins as an IHC panel could divide patients into different subgroups (P<0.000001). Multivariate cox regression analysis indicated that the three-protein panel was an independent prognostic factor with very high statistical significance (HR = 2.090, 95% CI: 1.621-2.696, P = 0.00000001). The data suggest that the three-protein panel of PI3K-p85α, EGFR and p53 is an important candidate biomarker for the prognosis of patients with ESCC.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Biomarkers, Tumor / metabolism*
-
Carcinoma, Squamous Cell / metabolism*
-
Carcinoma, Squamous Cell / mortality
-
Carcinoma, Squamous Cell / surgery
-
Class Ia Phosphatidylinositol 3-Kinase / metabolism
-
ErbB Receptors / metabolism
-
Esophageal Neoplasms / metabolism*
-
Esophageal Neoplasms / mortality
-
Esophageal Neoplasms / surgery
-
Esophageal Squamous Cell Carcinoma
-
Female
-
Humans
-
Kaplan-Meier Estimate
-
Male
-
Middle Aged
-
Prognosis
-
Proportional Hazards Models
-
Proto-Oncogene Proteins c-kit / metabolism
-
Tissue Inhibitor of Metalloproteinase-1 / metabolism
-
Tumor Suppressor Protein p53 / metabolism
Substances
-
Biomarkers, Tumor
-
TIMP1 protein, human
-
TP53 protein, human
-
Tissue Inhibitor of Metalloproteinase-1
-
Tumor Suppressor Protein p53
-
Class Ia Phosphatidylinositol 3-Kinase
-
EGFR protein, human
-
ErbB Receptors
-
Proto-Oncogene Proteins c-kit
Grants and funding
This work was supported by the National High Technology Research and Development Program of China (2012AA02A503, 2011YQ17006710) and National Natural Science Foundation of China (81330052). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.