Syndecan-1 regulates adipogenesis: new insights in dedifferentiated liposarcoma tumorigenesis

Carcinogenesis. 2015 Jan;36(1):32-40. doi: 10.1093/carcin/bgu222. Epub 2014 Oct 24.

Abstract

Syndecan-1 (SDC1/CD138) is one of the main cell surface proteoglycans and is involved in crucial biological processes. Only a few studies have analyzed the role of SDC1 in mesenchymal tumor pathogenesis. In particular, its involvement in adipose tissue tumors has never been investigated. Dedifferentiated liposarcoma, one of the most frequent types of malignant adipose tumors, has a high potential of recurrence and metastastic evolution. Classical chemotherapy is inefficient in metastatic dedifferentiated liposarcoma and novel biological markers are needed for improving its treatment. In this study, we have analyzed the expression of SDC1 in well-differentiated/dedifferentiated liposarcomas and showed that SDC1 is highly overexpressed in dedifferentiated liposarcoma compared with normal adipose tissue and lipomas. Silencing of SDC1 in liposarcoma cells impaired cell viability and proliferation. Using the human multipotent adipose-derived stem cell model of human adipogenesis, we showed that SDC1 promotes proliferation of undifferentiated adipocyte progenitors and inhibits their adipogenic differentiation. Altogether, our results support the hypothesis that SDC1 might be involved in liposarcomagenesis. It might play a prominent role in the dedifferentiation process occurring when well-differentiated liposarcoma progress to dedifferentiated liposarcoma. Targeting SDC1 in these tumors might provide a novel therapeutic strategy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipogenesis*
  • Adipose Tissue / metabolism
  • Adipose Tissue / pathology*
  • Blotting, Western
  • Cell Differentiation*
  • Cell Proliferation
  • Cell Transformation, Neoplastic / genetics
  • Cell Transformation, Neoplastic / metabolism
  • Cell Transformation, Neoplastic / pathology*
  • Cells, Cultured
  • Flow Cytometry
  • Humans
  • Immunoenzyme Techniques
  • Liposarcoma / genetics
  • Liposarcoma / metabolism
  • Liposarcoma / pathology*
  • RNA, Messenger / genetics
  • RNA, Small Interfering / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells / metabolism
  • Stem Cells / pathology
  • Syndecan-1 / antagonists & inhibitors
  • Syndecan-1 / genetics
  • Syndecan-1 / metabolism*

Substances

  • RNA, Messenger
  • RNA, Small Interfering
  • SDC1 protein, human
  • Syndecan-1