Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene-containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high-throughput studies of disease-specific T cell responses.
Keywords: bioorthogonal chemistry; cleavable linkers; immunology; major histocompatibility complex; peptidomimetics.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.