Primary structure of the major O-glycosidically linked carbohydrate unit of human von Willebrand factor

B Samor; J C Michalski; C Mazurier; M Goudemand; P De Waard; J F Vliegenthart; G Strecker; J Montreuil

doi:10.1007/BF01047846

Primary structure of the major O-glycosidically linked carbohydrate unit of human von Willebrand factor

Glycoconj J. 1989;6(3):263-70. doi: 10.1007/BF01047846.

Authors

B Samor¹, J C Michalski, C Mazurier, M Goudemand, P De Waard, J F Vliegenthart, G Strecker, J Montreuil

Affiliation

¹ Centre Régional de Transfusion Sanguine, laboratoire d'Hémostase, Lille, France.

PMID: 2535488
DOI: 10.1007/BF01047846

Abstract

A reduced tetrasaccharide chain was obtained from human von Willebrand factor (vWF) by mild alkaline borohydride treatment. The purification of this O-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin A and Lens culinaris agglutinin and finally gel filtration. Its structure was determined by a combination of methylation studies and 500 MHz 1H-NMR spectroscopy to be: NeuAc(alpha 2-3)Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Affinity
Chromatography, Gel
Chromatography, Thin Layer
Glycoproteins / chemistry*
Glycoproteins / isolation & purification
Glycosides
Humans
Lectins
Magnetic Resonance Spectroscopy
Methylation
Molecular Sequence Data
Oligosaccharides / chemistry*
Oligosaccharides / isolation & purification
von Willebrand Factor / chemistry*
von Willebrand Factor / isolation & purification

Substances

Glycoproteins
Glycosides
Lectins
Oligosaccharides
von Willebrand Factor