Complete genome sequence of an emerging genotype of tobacco streak virus in the United States

Chellappan Padmanabhan; Shan Gao; Rugang Li; Shouan Zhang; Zhangjun Fei; Kai-Shu Ling

doi:10.1128/genomeA.01138-14

Complete genome sequence of an emerging genotype of tobacco streak virus in the United States

Genome Announc. 2014 Nov 6;2(6):e01138-14. doi: 10.1128/genomeA.01138-14.

Authors

Chellappan Padmanabhan¹, Shan Gao², Rugang Li¹, Shouan Zhang³, Zhangjun Fei, Kai-Shu Ling⁴

Affiliations

¹ Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, South Carolina, USA.
² Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York, USA.
³ Tropical Research & Education Center and Department of Plant Pathology, University of Florida, IFAS, Homestead, Florida, USA.
⁴ Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, South Carolina, USA [email protected].

Abstract

We report here the complete genome sequence of an emerging genotype of tobacco streak virus (TSV) infecting zucchini squash in Florida (TSV_FL13-07), obtained using deep sequencing of short RNAs (sRNAs) and validation by Sanger sequencing. TSV_FL13-07 shares only <90% sequence identity in all three genomic RNAs to several known U.S. isolates.