Grease matrix as a versatile carrier of proteins for serial crystallography

Nat Methods. 2015 Jan;12(1):61-3. doi: 10.1038/nmeth.3172. Epub 2014 Nov 10.

Abstract

Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases / chemistry
  • Crystallization
  • Crystallography, X-Ray / methods*
  • Fatty Acid Binding Protein 3
  • Fatty Acid-Binding Proteins / chemistry
  • Lasers
  • Lubricants*
  • Mineral Oil
  • Muramidase / chemistry
  • Plant Proteins / chemistry
  • Proteins / chemistry*

Substances

  • FABP3 protein, human
  • Fatty Acid Binding Protein 3
  • Fatty Acid-Binding Proteins
  • Lubricants
  • Plant Proteins
  • Proteins
  • thaumatin protein, plant
  • Mineral Oil
  • Muramidase
  • Aldose-Ketose Isomerases
  • xylose isomerase

Associated data

  • PDB/3WUL
  • PDB/3WUM
  • PDB/3WXQ
  • PDB/3WXS
  • PDB/3WXT
  • PDB/3WXU
  • PDB/4W4Q