The assessment of the individual ability of modulating and coordinating the right and left bite force is poorly investigated. The present study describes a methodology for the assessment of the bilateral control of the biting force and evaluates the test-retest reliability in a sample of 13 healthy subjects. By modulating the intensity and the left/right balance of the biting force, the subject was able to drive a cursor on the screen to "reach and hold" targets, randomly generated within the physiological "range of force" of the subject. The average motor performance was evaluated by the mean cursor-target distance = 13 ± 5%, the Offset Error = 9 ± 5% and the standard deviation of the force vector = 17.7 ± 6.1% (expressed as % of the target). Mean distance and standard deviation indices had acceptable reliability. This technique improves the characterization of the mandibular motor function and it may have a relevant role for the assessment and rehabilitation of the neuromusculoskeletal disorders affecting the orofacial system.