Abstract
We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) for the identification of Vibrio cholerae. MS identified all 42 isolates of V. cholerae O1 and O139 and 7 of 9 non-O1/O139 isolates. MS correctly discriminated between all Aeromonas and V. cholerae isolates. Overall, MS performed as well as or better than biochemical methods.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Typing Techniques / methods*
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Cholera / diagnosis*
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Cholera / microbiology
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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Humans
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Molecular Typing / methods*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
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Vibrio cholerae / chemistry*
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Vibrio cholerae / classification
Substances
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Bacterial Proteins
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DNA, Bacterial