Kinetics of BMP-2 release from collagen carrier: evaluation by enzyme immunoassay in the presence of plasma proteins

E O Osidak; M S Osidak; D E Sivogrivov; T S Portnaya; T M Grunina; Z M Galushkina; V G Lunin; A S Karyagina; S P Domogatskii

doi:10.1007/s10517-014-2702-y

Kinetics of BMP-2 release from collagen carrier: evaluation by enzyme immunoassay in the presence of plasma proteins

Bull Exp Biol Med. 2014 Nov;158(1):104-8. doi: 10.1007/s10517-014-2702-y. Epub 2014 Nov 19.

Authors

E O Osidak¹, M S Osidak, D E Sivogrivov, T S Portnaya, T M Grunina, Z M Galushkina, V G Lunin, A S Karyagina, S P Domogatskii

Affiliation

¹ Laboratory of Bioactive Nanostructures, N. F. Gamaleya Research Institute of Epidemiology and Epidemiology, Ministry of Health of the Russian Federation, Moscow, Russia, [email protected].

PMID: 25403408
DOI: 10.1007/s10517-014-2702-y

Abstract

Test system ELISA-BMP-2 is developed for measuring recombinant human bone morphogenetic protein-2 in human and laboratory animal serum and plasma by sandwich ELISA. The test system has been used for studies of the kinetics of bone morphogenetic protein-2 release from collagen carrier in the presence of plasma proteins.

MeSH terms

Animals
Blood Proteins / chemistry*
Bone Morphogenetic Protein 2 / chemistry*
Collagen / chemistry*
Colloids
Drug Carriers / chemistry*
Enzyme-Linked Immunosorbent Assay
Humans
Hydrogels
Kinetics
Rabbits
Rats

Substances

BMP2 protein, human
Blood Proteins
Bone Morphogenetic Protein 2
Colloids
Drug Carriers
Hydrogels
Collagen