Antibodies specific to the retinoic acid human nuclear receptors alpha and beta

M P Gaub; Y Lutz; E Ruberte; M Petkovich; N Brand; P Chambon

doi:10.1073/pnas.86.9.3089

Antibodies specific to the retinoic acid human nuclear receptors alpha and beta

Proc Natl Acad Sci U S A. 1989 May;86(9):3089-93. doi: 10.1073/pnas.86.9.3089.

Authors

M P Gaub¹, Y Lutz, E Ruberte, M Petkovich, N Brand, P Chambon

Affiliation

¹ Unité 184 de Biologie Moléculaire et de Génie Génétique de Institut National de la Santé et de la Recherche Médicale, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.

Abstract

Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 and SP248). The specificity of these antisera was confirmed both by immunocytochemical detection of these receptors in COS-1 cells transfected with RAR-alpha and RAR-beta expression vectors and by immunoblot analyses performed with whole extracts of these cells. We also demonstrate that these antisera recognize RAR-alpha and RAR-beta endogenously expressed in the RA-responsive human promyelocytic leukemia cell line HL-60.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antibodies / immunology*
Antibody Specificity
Blotting, Western
Carrier Proteins / analysis
Carrier Proteins / genetics
Carrier Proteins / immunology*
DNA / genetics
Electrophoresis, Polyacrylamide Gel
HeLa Cells
Humans
Immunohistochemistry
Leukemia, Promyelocytic, Acute / metabolism
Molecular Sequence Data
Peptide Fragments / immunology
Receptors, Retinoic Acid
Transfection
Tumor Cells, Cultured

Substances

Antibodies
Carrier Proteins
Peptide Fragments
Receptors, Retinoic Acid
DNA