Antibody landscapes after influenza virus infection or vaccination

J M Fonville; S H Wilks; S L James; A Fox; M Ventresca; M Aban; L Xue; T C Jones; N M H Le; Q T Pham; N D Tran; Y Wong; A Mosterin; L C Katzelnick; D Labonte; T T Le; G van der Net; E Skepner; C A Russell; T D Kaplan; G F Rimmelzwaan; N Masurel; J C de Jong; A Palache; W E P Beyer; Q M Le; T H Nguyen; H F L Wertheim; A C Hurt; A D M E Osterhaus; I G Barr; R A M Fouchier; P W Horby; D J Smith

doi:10.1126/science.1256427

Antibody landscapes after influenza virus infection or vaccination

Science. 2014 Nov 21;346(6212):996-1000. doi: 10.1126/science.1256427.

Authors

J M Fonville^#^{1

2

3}, S H Wilks^#^{1

2}, S L James^{1

2}, A Fox⁴, M Ventresca¹, M Aban⁵, L Xue⁵, T C Jones^{1

2}, N M H Le⁴, Q T Pham⁶, N D Tran⁶, Y Wong⁷, A Mosterin^{1

2}, L C Katzelnick^{1

2}, D Labonte⁸, T T Le⁶, G van der Net³, E Skepner^{1

2}, C A Russell^{2

9}, T D Kaplan¹⁰, G F Rimmelzwaan³, N Masurel³, J C de Jong³, A Palache¹¹, W E P Beyer³, Q M Le⁶, T H Nguyen⁶, H F L Wertheim^{4

12}, A C Hurt^{5

13}, A D M E Osterhaus³, I G Barr⁵, R A M Fouchier³, P W Horby^{4

12}, D J Smith^{1

2

3}

Affiliations

¹ Center for Pathogen Evolution, Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
² WHO Collaborating Center for Modeling, Evolution, and Control of Emerging Infectious Diseases, Cambridge CB2 3EJ, UK.
³ Department of Viroscience, Erasmus Medical Center, Rotterdam 3015 CE, the Netherlands.
⁴ Oxford University Clinical Research Unit and Wellcome Trust Major Overseas Programme, Hanoi, Vietnam.
⁵ WHO Collaborating Centre for Reference and Research on Influenza, VIDRL at the Peter Doherty Institute for Infection and Immunity, Melbourne VIC 3000, Australia.
⁶ National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
⁷ Oxford University Museum of Natural History, Oxford OX1 3PW, UK.
⁸ Insect Biomechanics Group, Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
⁹ Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
¹⁰ bobblewire.com, Saint Louis, MO 63112, US.
¹¹ Abbott Laboratories, Weesp 1380 DA, the Netherlands.
¹² Nuffield Department of Clinical Medicine, Centre for Tropical Medicine, University of Oxford, Oxford OX3 7BN, UK.
¹³ Melbourne School of Population and Global Health, University of Melbourne, Parkville VIC 3010, Australia.

^# Contributed equally.

Abstract

We introduce the antibody landscape, a method for the quantitative analysis of antibody-mediated immunity to antigenically variable pathogens, achieved by accounting for antigenic variation among pathogen strains. We generated antibody landscapes to study immune profiles covering 43 years of influenza A/H3N2 virus evolution for 69 individuals monitored for infection over 6 years and for 225 individuals pre- and postvaccination. Upon infection and vaccination, titers increased broadly, including previously encountered viruses far beyond the extent of cross-reactivity observed after a primary infection. We explored implications for vaccination and found that the use of an antigenically advanced virus had the dual benefit of inducing antibodies against both advanced and previous antigenic clusters. These results indicate that preemptive vaccine updates may improve influenza vaccine efficacy in previously exposed individuals.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / blood
Antibodies, Viral / immunology*
Antigenic Variation / genetics
Antigenic Variation / immunology
Evolution, Molecular
Humans
Influenza A Virus, H3N2 Subtype / genetics
Influenza A Virus, H3N2 Subtype / immunology*
Influenza Vaccines / immunology*
Influenza, Human / blood
Influenza, Human / immunology*
Influenza, Human / prevention & control
Vaccination*

Substances

Antibodies, Viral
Influenza Vaccines

Abstract

Publication types

MeSH terms

Substances

Grants and funding