A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval

Kyung Jin Son; Dong-Sik Shin; Timothy Kwa; Jungmok You; Yandong Gao; Alexander Revzin

doi:10.1039/c4lc00884g

A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval

Lab Chip. 2015 Feb 7;15(3):637-41. doi: 10.1039/c4lc00884g.

Authors

Kyung Jin Son¹, Dong-Sik Shin, Timothy Kwa, Jungmok You, Yandong Gao, Alexander Revzin

Affiliation

¹ Department of Biomedical Engineering, University of California, Davis, USA. [email protected].

PMID: 25421651
DOI: 10.1039/c4lc00884g

Abstract

We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antibodies / immunology
CD4 Antigens / immunology
Fluorescence Resonance Energy Transfer
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
Microfluidic Analytical Techniques / instrumentation*
Microfluidic Analytical Techniques / methods*
Particle Size
Peptides / chemistry
Photochemical Processes
Single-Cell Analysis / instrumentation*
Single-Cell Analysis / methods*
Surface Properties
U937 Cells

Substances

Antibodies
CD4 Antigens
Peptides
Hydrogel, Polyethylene Glycol Dimethacrylate