High level of plasma matrix metalloproteinase-11 is associated with clinicopathological characteristics in patients with oral squamous cell carcinoma

Chung-Han Hsin; Mu-Kuan Chen; Chih-Hsin Tang; Huang-Pin Lin; Ming-Yung Chou; Chiao-Wen Lin; Shun-Fa Yang

doi:10.1371/journal.pone.0113129

High level of plasma matrix metalloproteinase-11 is associated with clinicopathological characteristics in patients with oral squamous cell carcinoma

PLoS One. 2014 Nov 25;9(11):e113129. doi: 10.1371/journal.pone.0113129. eCollection 2014.

Authors

Chung-Han Hsin¹, Mu-Kuan Chen², Chih-Hsin Tang³, Huang-Pin Lin¹, Ming-Yung Chou⁴, Chiao-Wen Lin⁴, Shun-Fa Yang⁵

Affiliations

¹ School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan.
² Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.
³ Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Department of Biotechnology, College of Health Science, Asia University, Taichung, Taiwan.
⁴ Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan.
⁵ Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.

Abstract

Background: Matrix metalloproteinase-11 (MMP-11) is reported to be overexpressed in several cancers and may contribute to tumorigenesis. The current study investigated the association between the clinicopathological characteristics and plasma level of MMP-11 in oral squamous cell carcinoma (OSCC) patients.

Methodology and principal findings: The plasma MMP-11 concentration was determined by ELISA on 330 male OSCC patients. In addition, the metastatic effects of the MMP-11 knockdown on the oral cancer cells were investigated by cell migration assay. Our results showed that the plasma MMP-11 levels were significantly higher in patients with advanced T status (p = 0.001), lymph node metastasis (p = 0.006) and higher TNM stages (p<0.001). Moreover, treatment with the MMP-11 shRNA exerted an inhibitory effect on migration in SCC9 oral cancer cells.

Conclusion: Our study showed that plasma level of MMP-11 may be useful for assessment of the disease progression, especially lymph node metastasis, in patients with OSCC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Biomarkers, Tumor / blood*
Blotting, Western
Carcinoma, Squamous Cell / enzymology*
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Enzyme-Linked Immunosorbent Assay
Gene Knockdown Techniques
Humans
Male
Matrix Metalloproteinase 11 / blood*
Matrix Metalloproteinase 11 / genetics
Middle Aged
Mouth Neoplasms / enzymology*
Mouth Neoplasms / pathology
Real-Time Polymerase Chain Reaction

Substances

Biomarkers, Tumor
Matrix Metalloproteinase 11

Grants and funding

This study was supported by a research grant from National Science Council, Taiwan (NSC 101-2314-B-040-019), Health and welfare surcharge of tobacco products, Ministry of Health and Welfare (MOHW103-TD-B-111-08), Chung Shan Medical University Hospital, Taiwan (CSH-2014-C-001) and Chung Shan Medical University and Changhua Christian Hospital (CSMU-CCH-102-004). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.