Abstract
Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579T resD and resEgenes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD andResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.
MeSH terms
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Bacillus cereus / genetics*
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Bacillus cereus / metabolism
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Bacterial Proteins* / biosynthesis
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Bacterial Proteins* / chemistry
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Bacterial Proteins* / genetics
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Bacterial Proteins* / isolation & purification
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Recombinant Fusion Proteins* / biosynthesis
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Recombinant Fusion Proteins* / chemistry
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Recombinant Fusion Proteins* / genetics
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Recombinant Fusion Proteins* / isolation & purification
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Transcription Factors* / biosynthesis
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Transcription Factors* / chemistry
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Transcription Factors* / genetics
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Transcription Factors* / isolation & purification
Substances
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Bacterial Proteins
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Recombinant Fusion Proteins
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Transcription Factors