Structural and biochemical characterization of a carbohydrate acetylesterase from Sinorhizobium meliloti 1021

FEBS Lett. 2015 Jan 2;589(1):117-22. doi: 10.1016/j.febslet.2014.11.033. Epub 2014 Nov 29.

Abstract

In many microorganisms, carbohydrate acetylesterases remove the acetyl groups from various types of carbohydrates. Sm23 from Sinorhizobium meliloti is a putative member of carbohydrate esterase family 3 (CE3) in the CAZy classification system. Here, we determined the crystal structure of Sm23 at 1.75 Å resolution and investigated functional properties using biochemical methods. Furthermore, immobilized Sm23 exhibited improved stability compared with soluble Sm23, which can be used for the design of plant cell wall degrading-systems.

Keywords: Acetylation; Plant cell wall; Rhizobium species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylesterase / chemistry*
  • Acetylesterase / genetics
  • Acetylesterase / metabolism
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Crystallography, X-Ray
  • Enzyme Stability
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / genetics
  • Enzymes, Immobilized / metabolism
  • Sinorhizobium meliloti / enzymology*
  • Sinorhizobium meliloti / genetics

Substances

  • Bacterial Proteins
  • Enzymes, Immobilized
  • Acetylesterase