Abstract
Synthetic peptides, 2 derived from the sequence common to small, middle and large T-antigen, and I derived from the sequence unique for middle T, activated lymphocytes from polyoma-virus-immunized, but not from control mice, to release the lymphokine migration inhibitory factor (MIF). In contrast, purified, bacterially grown, full-length small T-antigen and a middle T-antigen mutant Py 1387T MT (lacking 37 C-terminal amino acids) did not induce lymphokine release, although they were capable of inducing an anti-polyoma TSTA response in vivo.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Polyomavirus Transforming / immunology*
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Antigens, Polyomavirus Transforming / isolation & purification
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Cell Migration Inhibition*
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Immunization / methods
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Immunization Schedule
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Macrophage Activation / drug effects
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Macrophage Migration-Inhibitory Factors / biosynthesis
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Macrophages / drug effects*
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Macrophages / immunology
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Mice
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Mice, Inbred CBA
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Peptides / chemical synthesis
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Peptides / pharmacology*
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Polyomavirus / immunology*
Substances
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Antigens, Polyomavirus Transforming
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Macrophage Migration-Inhibitory Factors
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Peptides