The effects of D-allose on transient ischemic neuronal death and analysis of its mechanism

Brain Res Bull. 2014 Oct:109:127-31. doi: 10.1016/j.brainresbull.2014.10.005. Epub 2014 Oct 14.

Abstract

The present study investigates the neuroprotective effects of d-allose, a rare sugar, against ischemia/reperfusion injury in a gerbil model. Transient forebrain ischemia was induced by occlusion of the bilateral common carotid arteries for 5 min. D-Allose was intravenously injected before and after ischemia (200 mg/kg). Extracellular glutamate and lactate release from the gerbil brain, and PO₂ profiles were monitored during ischemia and reperfusion. We also examined neuronal death and oxidative damage in the hippocampus one week after ischemia reperfusion, and investigated functional outcome. D-Allose administration suppressed glutamate and lactate release compared to vehicle controls. Brain damage, 8-OHdG levels (a marker of oxidative stress) and locomotor activities were significantly decreased by D-allose treatment. The present results suggest that d-allose reduces delayed neuronal death and behavioral deficits after transient ischemia by changing cerebral metabolism and inhibiting oxidative stress.

Keywords: Glutamate; Lactate; Oxidation; Reperfusion; Transient forebrain ischemia; d-Allose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Analysis of Variance
  • Animals
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Cerebral Cortex / pathology
  • DNA Damage / drug effects
  • Deoxyguanosine / analogs & derivatives
  • Disease Models, Animal
  • Drug Administration Schedule
  • Gerbillinae
  • Glucose / pharmacology*
  • Glucose / therapeutic use
  • Glutamic Acid / metabolism
  • Ischemic Attack, Transient / complications
  • Ischemic Attack, Transient / drug therapy
  • Ischemic Attack, Transient / pathology*
  • Lactic Acid / metabolism
  • Male
  • Microdialysis
  • Movement Disorders / etiology
  • Neurons / drug effects*
  • Oxygen / metabolism
  • Time Factors

Substances

  • Lactic Acid
  • Glutamic Acid
  • allose
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine
  • Glucose
  • Oxygen