α-Synuclein modifies mutant huntingtin aggregation and neurotoxicity in Drosophila

Hum Mol Genet. 2015 Apr 1;24(7):1898-907. doi: 10.1093/hmg/ddu606. Epub 2014 Dec 1.

Abstract

Protein misfolding and aggregation is a major hallmark of neurodegenerative disorders such as Alzheimer's disease (AD), Parkinson's disease (PD) and Huntington's disease (HD). Until recently, the consensus was that each aggregation-prone protein was characteristic of each disorder [α-synuclein (α-syn)/PD, mutant huntingtin (Htt)/HD, Tau and amyloid beta peptide/AD]. However, growing evidence indicates that aggregation-prone proteins can actually co-aggregate and modify each other's behavior and toxicity, suggesting that this process may also contribute to the overlap in clinical symptoms across different diseases. Here, we show that α-syn and mutant Htt co-aggregate in vivo when co-expressed in Drosophila and produce a synergistic age-dependent increase in neurotoxicity associated to a decline in motor function and life span. Altogether, our results suggest that the co-existence of α-syn and Htt in the same neuronal cells worsens aggregation-related neuropathologies and accelerates disease progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Disease Models, Animal
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila / metabolism
  • Female
  • Humans
  • Huntingtin Protein
  • Male
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Nerve Tissue Proteins / toxicity
  • Neurodegenerative Diseases / genetics
  • Neurodegenerative Diseases / metabolism*
  • Protein Aggregates
  • alpha-Synuclein / genetics
  • alpha-Synuclein / metabolism*

Substances

  • HTT protein, human
  • Huntingtin Protein
  • Nerve Tissue Proteins
  • Protein Aggregates
  • alpha-Synuclein