Alarmins MRP8 and MRP14 induce stress tolerance in phagocytes under sterile inflammatory conditions

Cell Rep. 2014 Dec 24;9(6):2112-23. doi: 10.1016/j.celrep.2014.11.020. Epub 2014 Dec 11.

Abstract

Hyporesponsiveness by phagocytes is a well-known phenomenon in sepsis that is frequently induced by low-dose endotoxin stimulation of Toll-like receptor 4 (TLR4) but can also be found under sterile inflammatory conditions. We now demonstrate that the endogenous alarmins MRP8 and MRP14 induce phagocyte hyporesponsiveness via chromatin modifications in a TLR4-dependent manner that results in enhanced survival to septic shock in mice. During sterile inflammation, polytrauma and burn trauma patients initially present with high serum concentrations of myeloid-related proteins (MRPs). Human neonatal phagocytes are primed for hyporesponsiveness by increased peripartal MRP concentrations, which was confirmed in murine neonatal endotoxinemia in wild-type and MRP14(-/-) mice. Our data therefore indicate that alarmin-triggered phagocyte tolerance represents a regulatory mechanism for the susceptibility of neonates during systemic infections and sterile inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Burns / immunology
  • Burns / metabolism
  • Calgranulin A / blood
  • Calgranulin A / genetics
  • Calgranulin A / metabolism*
  • Calgranulin B / blood
  • Calgranulin B / genetics
  • Calgranulin B / metabolism*
  • Cell Line
  • Cells, Cultured
  • Chromatin Assembly and Disassembly
  • Female
  • Humans
  • Immune Tolerance*
  • Inflammation / metabolism
  • Male
  • Mice
  • Middle Aged
  • NF-kappa B / metabolism
  • Phagocytes / immunology
  • Phagocytes / metabolism*
  • Shock, Septic / immunology
  • Shock, Septic / metabolism
  • Stress, Physiological

Substances

  • Calgranulin A
  • Calgranulin B
  • NF-kappa B

Associated data

  • GEO/GSE61477