Chimeric neuraminidase and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus

Ewan P Plant; Zhiping Ye

doi:10.1099/jgv.0.000025

Chimeric neuraminidase and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus

J Gen Virol. 2015 Apr;96(Pt 4):752-755. doi: 10.1099/jgv.0.000025. Epub 2014 Dec 12.

Authors

Ewan P Plant¹, Zhiping Ye¹

Affiliation

¹ Laboratory of Respiratory Viral Diseases, Division of Viral Products, Office of Vaccine Research and Review, US Food and Drug Administration, Silver Spring, MD 20993, USA.

PMID: 25502649
DOI: 10.1099/jgv.0.000025

Abstract

We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here, we showed that the same mutations improved the growth kinetics of a virus containing the A/Vietnam/1203/2004 (H5N1) haemagglutinin and neuraminidase (NA). Total protein yield and NA activity were increased when a chimeric NA was included. These increases indicated that the synergistic effect was due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.

Publication types

Editorial
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Coculture Techniques
Dogs
HEK293 Cells
Hemagglutinins / genetics
Humans
Influenza A Virus, H5N1 Subtype / enzymology
Influenza A Virus, H5N1 Subtype / genetics*
Influenza A Virus, H5N1 Subtype / growth & development*
Influenza A Virus, H5N1 Subtype / immunology
Influenza Vaccines / genetics*
Influenza Vaccines / immunology
Madin Darby Canine Kidney Cells
Mutation*
Neuraminidase / genetics*
Viral Proteins / genetics*

Substances

Hemagglutinins
Influenza Vaccines
Viral Proteins
influenza virus polymerase basic protein 1
Neuraminidase