Inhibition of fibrin polymerization by synthetic peptides corresponding to Aα195-205 and γ69-77 sites of fibrin molecule

Ukr Biochem J. 2014 Jul-Aug;86(4):119-25. doi: 10.15407/ubj86.04.119.

Abstract

Using the idea of "proline brackets" we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Aα195-205 specifically inhibited the stage of fibrin protofibril formation and peptide γ69-77 - the stage offibrin protofibril lateral association. The data obtained testify that there are the sites involved in these processes in the appropriate amino acid sequences of fibrin molecule.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Blood Coagulation / drug effects
  • Fibrin / chemistry*
  • Fibrinogen / chemistry
  • Humans
  • Peptide Fragments / chemistry*
  • Peptide Fragments / pharmacology*
  • Polymerization
  • Proline / chemistry*
  • Thrombin / chemistry

Substances

  • Peptide Fragments
  • Fibrin
  • Fibrinogen
  • Proline
  • Thrombin