Glycolysis controls plasma membrane glucose sensors to promote glucose signaling in yeasts

Amélie Cairey-Remonnay; Julien Deffaud; Micheline Wésolowski-Louvel; Marc Lemaire; Alexandre Soulard

doi:10.1128/MCB.00515-14

Glycolysis controls plasma membrane glucose sensors to promote glucose signaling in yeasts

Mol Cell Biol. 2015 Feb;35(4):747-57. doi: 10.1128/MCB.00515-14. Epub 2014 Dec 15.

Authors

Amélie Cairey-Remonnay¹, Julien Deffaud¹, Micheline Wésolowski-Louvel¹, Marc Lemaire¹, Alexandre Soulard²

Affiliations

¹ Université Lyon 1, CNRS, UMR5240 Microbiologie, Adaptation et Pathogénie, équipe Génétique Moléculaire des Levures, Villeurbanne, France.
² Université Lyon 1, CNRS, UMR5240 Microbiologie, Adaptation et Pathogénie, équipe Génétique Moléculaire des Levures, Villeurbanne, France [email protected].

Abstract

Sensing of extracellular glucose is necessary for cells to adapt to glucose variation in their environment. In the respiratory yeast Kluyveromyces lactis, extracellular glucose controls the expression of major glucose permease gene RAG1 through a cascade similar to the Saccharomyces cerevisiae Snf3/Rgt2/Rgt1 glucose signaling pathway. This regulation depends also on intracellular glucose metabolism since we previously showed that glucose induction of the RAG1 gene is abolished in glycolytic mutants. Here we show that glycolysis regulates RAG1 expression through the K. lactis Rgt1 (KlRgt1) glucose signaling pathway by targeting the localization and probably the stability of Rag4, the single Snf3/Rgt2-type glucose sensor of K. lactis. Additionally, the control exerted by glycolysis on glucose signaling seems to be conserved in S. cerevisiae. This retrocontrol might prevent yeasts from unnecessary glucose transport and intracellular glucose accumulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Cell Membrane
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Feedback, Physiological
Fungal Proteins / genetics
Fungal Proteins / metabolism
Gene Expression Regulation, Fungal*
Glucose / metabolism*
Glycolysis / genetics*
Isoenzymes / genetics
Isoenzymes / metabolism
Kluyveromyces / genetics
Kluyveromyces / metabolism*
Monosaccharide Transport Proteins / genetics
Monosaccharide Transport Proteins / metabolism
Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism
Sequence Homology, Amino Acid
Signal Transduction
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

DNA-Binding Proteins
Fungal Proteins
Isoenzymes
Monosaccharide Transport Proteins
RAG4 protein, Kluyveromyces
RGT1 protein, S cerevisiae
RGT2 protein, S cerevisiae
SNF3 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Phosphoenolpyruvate Sugar Phosphotransferase System
phosphoenolpyruvate-glucose phosphotransferase
Glucose