Complete Genome Sequence of Bifidobacterium longum 105-A, a Strain with High Transformation Efficiency

Yu Kanesaki; Hisayoshi Masutani; Mikiyasu Sakanaka; Yuh Shiwa; Takatomo Fujisawa; Yasukazu Nakamura; Atsushi Yokota; Satoru Fukiya; Tohru Suzuki; Hirofumi Yoshikawa

doi:10.1128/genomeA.01311-14

Complete Genome Sequence of Bifidobacterium longum 105-A, a Strain with High Transformation Efficiency

Genome Announc. 2014 Dec 18;2(6):e01311-14. doi: 10.1128/genomeA.01311-14.

Authors

Yu Kanesaki¹, Hisayoshi Masutani², Mikiyasu Sakanaka³, Yuh Shiwa¹, Takatomo Fujisawa⁴, Yasukazu Nakamura⁴, Atsushi Yokota³, Satoru Fukiya⁵, Tohru Suzuki⁶, Hirofumi Yoshikawa

Affiliations

¹ Genome Research Center, Tokyo University of Agriculture, Setagaya, Tokyo, Japan.
² Graduate School of Applied Biological Sciences, Gifu University, Gifu, Gifu, Japan.
³ Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
⁴ Center for Information Biology, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, Japan.
⁵ Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan [email protected] [email protected].
⁶ the United Graduate School of Agricultural Science, Gifu University, Gifu, Gifu, Japan.

Abstract

Bifidobacterium longum 105-A shows high transformation efficiency and allows for the generation of gene knockout mutants through homologous recombination. Here, we report the complete genome sequence of strain 105-A. Genes encoding at least four putative restriction-modification systems were found in this genome, which might contribute to its transformation efficiency.