Glycogen-enzymes containing bodies in type 2 fibres of tenotomized muscles in the rat

Gegenbaurs Morphol Jahrb. 1989;135(3):519-28.

Abstract

Inclusion bodies containing glycogen-enzymes were found in 30 to 60% of type 2 fibres of tenotomized calf muscles (m. gastrocnemius, m. soleus, m. plantaris) in rats, using histochemical reactions. The bodies appeared within 1 week after the tenotomy and were localized both in the central and the subsarcolemmal regions and rarely extruded into the extracellular space. These aggregates are 3 to 15 microns in length and 2 to 11 microns in diameter. In addition to glycogen, these bodies also contained various enzymes of the glycogen metabolism such as phosphorylase, a branching enzyme, and glucose-6-phosphatase, but showed no NADH-reductase, lactate dehydrogenase, or myofibrillar ATP-ase activity. The results indicate that glycogen-enzymes containing bodies are a degenerative phenomenon, which occurs only in type 2 fibres of the tenotomized muscles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,4-alpha-Glucan Branching Enzyme / analysis
  • Animals
  • Female
  • Glucose-6-Phosphatase / analysis
  • Glycogen / analysis*
  • Histocytochemistry
  • Inclusion Bodies / enzymology*
  • Muscles / enzymology*
  • Phosphorylases / analysis
  • Rats
  • Rats, Inbred Strains / anatomy & histology*

Substances

  • Glycogen
  • Phosphorylases
  • 1,4-alpha-Glucan Branching Enzyme
  • Glucose-6-Phosphatase