Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells

Polina Mishel; Dmitrii Bychkov; Hannimari Kallio-Kokko; Miia Valkonen; Anu Kantele; Pirkko Mattila; Henrikki Almusa; Petri Jalovaara; Denis Kainov

doi:10.1128/genomeA.01349-14

Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells

Genome Announc. 2014 Dec 24;2(6):e01349-14. doi: 10.1128/genomeA.01349-14.

Authors

Polina Mishel¹, Dmitrii Bychkov¹, Hannimari Kallio-Kokko², Miia Valkonen³, Anu Kantele³, Pirkko Mattila¹, Henrikki Almusa¹, Petri Jalovaara¹, Denis Kainov⁴

Affiliations

¹ The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
² Helsinki University Hospital Laboratory, HUSLAB, Helsinki, Finland.
³ Helsinki University Central Hospital, HUCS, Helsinki, Finland.
⁴ The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland [email protected].

Abstract

Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability of influenza genomes should be taken into consideration during drug-sensitivity studies.