Abstract
The activity of liver 6-phosphofructo-2-kinase (PFK-2), the enzyme that catalyses the synthesis of fructose 2,6-bisphosphate, was markedly decreased in hypothyroid rats and partially restored after 3 days of treatment with triiodothyronine. The changes in PFK-2 activity were accompanied by parallel changes in enzyme content measured by immunotitration and in PFK-2 mRNA determined by dot blot and Northern blot hybridization with cDNA probes. It is concluded that thyroid hormone stimulates liver PFK-2 gene expression by a pre-translational mechanism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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DNA Probes
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Fructosediphosphates / metabolism
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Gene Expression Regulation, Enzymologic / drug effects*
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Hypothyroidism / enzymology
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Liver / enzymology
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Male
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Myocardium / enzymology
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Phosphofructokinase-2
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Phosphotransferases / genetics*
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RNA, Messenger / genetics
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Rats
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Rats, Inbred Strains
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Thyroid Hormones / pharmacology*
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Triiodothyronine / pharmacology
Substances
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DNA Probes
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Fructosediphosphates
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RNA, Messenger
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Thyroid Hormones
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Triiodothyronine
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Phosphotransferases
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Phosphofructokinase-2