New host factors important for respiratory syncytial virus (RSV) replication revealed by a novel microfluidics screen for interactors of matrix (M) protein

Mol Cell Proteomics. 2015 Mar;14(3):532-43. doi: 10.1074/mcp.M114.044107. Epub 2015 Jan 2.

Abstract

Although human respiratory syncytial virus (RSV) is the most common cause of bronchiolitis and pneumonia in infants and elderly worldwide, there is no licensed RSV vaccine or effective drug treatment available. The RSV Matrix protein plays key roles in virus life cycle, being found in the nucleus early in infection in a transcriptional inhibitory role, and later localizing in viral inclusion bodies before coordinating viral assembly and budding at the plasma membrane. In this study, we used a novel, high throughput microfluidics platform and custom human open reading frame library to identify novel host cell binding partners of RSV matrix. Novel interactors identified included proteins involved in host transcription regulation, the innate immunity response, cytoskeletal regulation, membrane remodeling, and cellular trafficking. A number of these interactions were confirmed by immunoprecipitation and cellular colocalization approaches. Importantly, the physiological significance of matrix interaction with the actin-binding protein cofilin 1, caveolae protein Caveolin 2, and the zinc finger protein ZNF502 was confirmed. siRNA knockdown of the host protein levels resulted in reduced RSV virus production in infected cells. These results have important implications for future antiviral strategies aimed at targets of RSV matrix in the host cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / metabolism*
  • Caveolin 2 / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism
  • Chlorocebus aethiops
  • Cofilin 1 / metabolism*
  • Gene Library
  • HEK293 Cells
  • Humans
  • Microfluidic Analytical Techniques / methods*
  • Nuclear Proteins / metabolism*
  • Open Reading Frames
  • Respiratory Syncytial Viruses / physiology*
  • Vero Cells
  • Viral Matrix Proteins / metabolism*
  • Virus Replication

Substances

  • CAV2 protein, human
  • CFL1 protein, human
  • Carrier Proteins
  • Caveolin 2
  • Cofilin 1
  • Nuclear Proteins
  • Viral Matrix Proteins
  • ZNF502 protein, human