Receptors for insulin and insulin-like growth factor-I in the human adrenal gland

Biochem Biophys Res Commun. 1989 Nov 30;165(1):204-11. doi: 10.1016/0006-291x(89)91055-3.

Abstract

Human adrenal glands contain high-affinity receptors for insulin and insulin-like growth factor I (IGF-I). Comparative studies with rat, hamster and human adrenal membranes confirmed that IGF-I receptors are most abundant in rat and hamster adrenals, whereas insulin and IGF-I receptors are present in equivalent numbers in human adrenal glands. Covalent crosslinking studies revealed that the human adrenal gland IGF-I receptor binding subunit migrated on dodecyl sulfate polyacrylamide gels with Mr = 135,000, which is identical to the migration of IGF-I receptor binding subunits isolated from other tissues. Autoradiography of frozen human adrenal slices incubated with [125I]insulin showed prominent, displaceable binding of this radioligand to the zona reticularis, zona glomerulosa, vasculature and medulla; in contrast, [125I]IGF-I binding to human adrenal tissue was most prominent in the zona reticularis and negligible in the medullary region.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenal Glands / metabolism*
  • Adrenal Glands / pathology
  • Autoradiography
  • Humans
  • Insulin-Like Growth Factor I / metabolism*
  • Iodine Radioisotopes
  • Kinetics
  • Postmortem Changes
  • Receptor, Insulin / metabolism*
  • Receptors, Cell Surface / metabolism*
  • Receptors, Somatomedin
  • Recombinant Proteins / metabolism
  • Somatomedins / metabolism*

Substances

  • Iodine Radioisotopes
  • Receptors, Cell Surface
  • Receptors, Somatomedin
  • Recombinant Proteins
  • Somatomedins
  • Insulin-Like Growth Factor I
  • Receptor, Insulin